The present book Detection and Diagnosis of Plant Diseases deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a ready made solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students. Table of Contents 1. Instruments used in the study of plant diseases/pathogens 2. Methods to Prove Koch’s postulates 3. Preparation of culture media for the growth and isolation of fungi and bacteria 4. Isolation of plant pathogens 5. Staining of fungal pathogens 6. Staining of bacterial pathogens 7. Isolation and purification of plant pathogenic fungi from rhizosphere soil 8. Isolation and purification of plant pathogenic bacteria from rhizosphere soil 9. Plant disease diagnosis: the complete process of field observations, sample collection, sample packaging and laboratory diagnosis 10. Identification of Plant Parasitic Nematode-Heterodera 11. Identification of Plant Parasitic Nematode-Meloidogyne 12. Identification of Plant Parasitic Nematode-Anguina 13. Extraction of Nematode From Soil by Sieving and Decantation Method 14. Sampling and Extraction of Nematodes From Diseased Plant Materials 15. Preparation of Nematode Mounting 16. Preservation of disease samples by dry and wet methods 17. Evaluation of fungicide toxicity by food poisoning method against pathogens 18. Important serological and molecular techniques and instruments in Plant Pathology Random Amplified Polymorphic DNA ( RAPD), PCR: Polymerase Chain Reaction Enzyme Linked Immune Sorbent Assay (ELISA) Dot- Immune Binding Assay (DIBA) Immunosorbent Electron Microscopy (ISEM) Isolation of fungal genomic DNA Restriction Fragment Length Polymorphism (RFLP) Amplified Fragment Length Polymorphism Centrifuge, Spectrophotometer, PH meter, Microscopy (Phase Contrast Microscopy), Chromatography, Camera Lucida 19. Preparation of buffer and reagents 20. Methods of biochemical analyses Estimation of Chlorophyll Estimation of Proline Estimation of Phenol Estimation of Protein Estimation of Total sugar (Reducing and Non-Reducing) Estimation of Polyphenol Oxidase (PPO) Estimation of Catalase (CAT) enzyme Estimation of Phenyl-alanine Ammonia Lyase (PAL) Estimation of Peroxidase (POD) Estimation of Superoxide Dismutase (SOD) 21. General Information Conversion tables and formula Plant Pathology Related Web Sites Important Symptoms of Plant Diseases Terminology Suggested Readings Composition of Different Media Used for Fungus and Bacterial Growth